柯荣秦 bwin必赢教授、博导

电子邮箱:E-mail:rke@hqu.edu.cn

-基本信息

柯荣秦 博士

bwin必赢教授、博导

E-mail:rke@hqu.edu.cn

柯荣秦博士,bwin必赢教授,入选国家级青年人才项目,福建省“闽江学者”特聘教授,厦门市首批杰出青年人才及“双百计划”领军型创业人才。曾就职于美国Complete Genomics公司,发明了基于DNA纳米球的双端测序技术,现为华大基因自主研发的国产二代测序系统的核心技术之一。RNA原位测序技术发明人,该技术入选了Nature Methods杂志2013年度的Method of the Year,是最早发表的空间转录组学技术。先后主持或参与国家自然科学基金、国家重点研发计划、福建省自然科学基金等国家级和省部级项目。

-教育背景

2001.09 – 2005.07 厦门大学生命科学学院生物科学学士

2005.09 – 2008.07 厦门大学生命科学学院生物化学与分子生物学硕士

2008.09 – 2012.12 瑞典乌普萨拉大学医学科学博士

-工作经历

2016.05 – 华侨大学生物医学学院教授

2015.01 – 2016.03 美国加州Complete Genomics, Inc. Senior Scientist

2013.11 – 2015. 01 美国加州Complete Genomics, Inc. Scientist

2013.01 – 2013.06 瑞典斯德哥尔摩Science for Life Laboratory博士后

-发表文章

1. Jiang M, Wei K, Li M, Lin C, Ke R. Single molecule RNA in situ detection in clinical FFPE tissue sections by vsmCISH. RNA. 2023.

2. Rao X, Zheng L, Wei K, Li M, Jiang M, Qiu J, Zhou Y, Ke R, Lin C. Novel In Situ Hybridization Assay for Chromogenic Single-Molecule Detection of Human Papillomavirus E6/E7 mRNA. Microbiology Spectrum. 2023.

3. Tang X, Chen J, Zhang X, Liu X, Xie Z, Wei K, Qiu J, Ma W, Lin C, Ke R. Improved in situ sequencing for high-resolution targeted spatial transcriptomic analysis in tissue sections. Journal of Genetics and Genomics. 2023.

4. Li Q, Zhang X, Ke R. Spatial Transcriptomics for Tumor Heterogeneity Analysis. Frontiers in Genetics. 2022, 13:906158.

5. Yin J, Lin C, Jiang M, Tang X, Xie D, Chen J, Ke R. CENPL, ISG20L2, LSM4, MRPL3 are four novel hub genes and may serve as diagnostic and prognostic markers in breast cancer. Sci Rep. 2021, 2, 11(1):15610.

6. Lin C, Liu X, Zheng B, Ke R, Tzeng CM. Liquid Biopsy, ctDNA Diagnosis through NGS. Life, 2021, 11(9):890.

7. Lin C, Jiang M, Liu L, Chen X, Zhao Y, Chen L, Hong Y, Wang X, Hong C, Yao X, Ke R. Imaging of individual transcripts by amplification-based single-molecule fluorescence in situ hybridization. New Biotechnology. 2021, 61:116-123.

8. Zhao Y, Lin C, Wu P, Chen X, Zhao Y, Li Y, Chen L, Nilsson M, Ke R. Single Cell RNA Expression Analysis Using Flow Cytometry Based on Specific Probe Ligation and Rolling Circle Amplification. ACS Sensors. 2020, 5, 10:3031–3036.

9. Lin C, Jiang M, Duan S, Qiu J, Hong Y, Wang X, Chen X, Ke R. Visualization of individual microRNA molecules in fixed cells and tissues using target-primed padlock probe assay. Biochem Biophys Res Commun. 2020. 526(3):607-611.

10. Jiang M, Liu L, Hong C, Chen D, Yao X, Chen X, Lin C, Ke R. Single molecule chromogenic in situ hybridization assay for RNA visualization in fixed cells and tissues. RNA. 2019 25(8):1038-1046.

11. Ke R, Mignardi M, Hauling T, Nilsson M. Fourth Generation of Next-Generation Sequencing Technologies: Promise and Consequences. Human Mutation. 2016, 37(12):1363-1367.

12. Ke R*, Mignardi M*, Botling J, Wählby C, and Nilsson M. In situ sequencing for RNA analysis in preserved cells and tissue. Nature Methods. 2013, 10: 857–860. *共同一作 (入选2013年度Method of the Year)

13. Ke R*, Nong RY*, Fredriksson S, Landegren U, Nilsson M. Improving precision of proximity ligation assay by amplified single molecule detection. PLoS One. 2013, 8(7): e69813. *共同一作

14. Gómez de la Torre TZ*, Ke R*, Mezger A, Svedlindh P, Strømme M, Nilsson M. Sensitive detection of spores using volume-amplified magnetic nanobeads. Small. 2012, 8:2174-7. *共同一作

15. Ke R, Zorzet A, Göransson J, Lindegren G, Sharifi-Mood B, Chinikar S, Mardani M, Mirazimi A, Nilsson M. Colorimetric nucleic acid testing assay for RNA virus detection based on circle-to-circle amplification of padlock probes. Journal of Clinical Microbiology. 2011, 49:4279-85.

16. Ke R*, Yang W*, Xia X, Xu Y, Li Q. Tandem conjugation of enzyme and antibody on silica nanoparticle for enzyme immunoassay. Analytical Biochemistry. 2010, 406:8-13. (selected for cover) *共同一作 (封面文章)

其它文章:

1. Zhang HX, Zhou YL, Xu WY, Chen XL, Jiang JY, Zhou XM, Wang ZG, Ke RQ, Guo QW. Polymerase chain reaction-based assays facilitate the breeding and study of mouse models of Klinefelter syndrome. Asian J Androl. 2023;53(1):76-80.

2. Zhang Q, Xia K, Jiang M, Li Q, Chen W, Han M, Li W, Ke R, Wang F, Zhao Y, Liu Y, Fan C, Gu H. Catalytic DNA-Assisted Mass Production of Arbitrary Single-Stranded DNA. Angew Chem Int Ed Engl. 2023, 62(5): e202212011.

3. Yu Y, Zeng Z, Xie D, Chen R, Sha Y, Huang S, Cai W, Chen W, Li W, Ke R, Sun T. Interneuron origin and molecular diversity in the human fetal brain. Nat Neurosci. 2021, 24(12):1745-1756.

4. Liu Q, Li J, Zhang W, Xiao C, Zhang S, Nian C, Li J, Su D, Chen L, Zhao Q, Shao H, Zhao H, Chen Q, Li Y, Geng J, Hong L, Lin S, Wu Q, Deng X, Ke R, Ding J, Johnson RL, Liu X, Chen L, Zhou D. Glycogen accumulation and phase separation drives liver tumor initiation. Cell. 2021, 184(22):5559-5576.e19.

5. Jiang M, Lin C, Ke R. Detection of Individual RNA in Fixed Cells and Tissues by Chromogenic ISH. Bio Protoc. 2020,10(3): e3510.

6. Zeng F, Ma X, Zhu L, Xu Q, Zeng Y, Gao Y, Li G, Guo T, Zhang H, Tang X, Wang Z, Ye Z, Zheng L, Zhang H, Zheng Q, Li K, Lu J, Qi X, Luo H, Zhang X, Wang Z, Zhou Y, Yao Y, Ke R, Zhou Y, Liu Y, Sun H, Huang T, Shao Z, Xu H, Wang X. The deubiquitinase USP6 affects memory and synaptic plasticity through modulating NMDA receptor stability. PLoS Biol. 2019. 17(12): e3000525.

7. New technologies for DNA analysis--a review of the READNA Project, New Biotechnology. 2016, 33:311-330.

8. Hauling T, Ke R, Krzywkowski T, Nilsson M. In situ detection of individual mRNA molecules with padlock probes and target-primed rolling-circle amplification in fixed mouse brain tissues. In Situ Hybridization Methods. 2015: 485-507.

9. Sun S, Ke R, Hughes D, Nilsson M, Andersson DI. Genome-Wide Detection of Spontaneous Chromosomal Rearrangements in Bacteria. PLoS One. 2012, 7: e42639.

10. Göransson J, Ke R, Nong RY, Howell WM, Karman A, Grawé J, Stenberg J, Granberg M, Elgh M, Herthnek D, Wikström P, Jarvius J, Nilsson M. Rapid identification of bio-molecules applied for detection of biosecurity agents using rolling circle amplification. PLoS One. 2012, 7: e31068.

11. Barišić I, Schoenthaler S, Ke R, Nilsson M, Noehammer C, Wiesinger-Mayr H, Multiplex detection of antibiotic resistance genes using padlock probes. Diagnostic microbiology and infectious disease. 2013, 77: 118-125.

12. Xia X, Xu Y, Ke R, Zhang H, Zou M, Yang W, Li Q, A highly sensitive europium nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue, Analytical Bioanalytical Chemistry. 2013, 45: 7541-7544.

13. Pacureanu A, Ke R, Mignardi M, Nilsson M, Wählby C, Image based in situ sequencing for RNA analysis in tissue. Biomedical Imaging (ISBI), IEEE 11th International Symposium on, 2014: 286-289.

-所获荣誉

福建省“闽江学者”特聘教授(2015年)

国家高层次人才计划(2016年)

厦门市首批杰出青年人才(2017年)

泉州市高层次人才(第二层次)(2018年)

福建省高校青年拔尖人才(2018年)

厦门市“双百计划”领军型创业人才(2019年)

福建省杰出青年基金(2022年)

地址:福建省泉州市丰泽区bwin必赢

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